Introduction: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.

Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (fsh). Description: Follistatin Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa. The FST is purified by proprietary chromatographic techniques. Source: Sterile Filtered White lyophilized (freeze-dried) powder. Catalog Number: CYT-232Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized from a concentrated (1mg/ml) solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized Follistatin in sterile 18MΩ -cm H2O not less than 100µ G/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution FST should be stored at 4° C between 2-7 days and for future use below -18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Asn-Cys-Trp-Leu. Biological Activity: The activity is determined by the ability to neutralize Activin A inhibitory effect of mouse MPC-11 cells. The expected ED50 is 0.1-0.4 µ G/ml in the presence of 7.5 ng/ml Activin A. Application: FST will rein in muscle growth statin (myostatin) and promote muscle fibers growth. Also can be used for muscle atrophy treatment research and muscular fitness research, animal breeding research field.